Free gamified quizzes on every subject that students play in class and at home. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then. There are three formulas used to work microbiology dilution problems. Bacterial dilutions and a foolproof way to figure them out. Producing samples with different concentrations in a series is more time efficient than trying to prepare each sample one by one.
Choose from 184 different sets of serial dilutions flashcards on quizlet. Bacterial dilutions and a foolproof way to figure them out look at the dilution scheme below. Learn serial dilution with free interactive flashcards. The following formula can easily be used to carry out a simple dilution. You will receive your score and answers at the end. Calculate the molarity of the resulting solution if a certain volume of water was added to 50. Dilutions worksheet solutions 1 if i have 340 ml of a 0. Worksheets are dilutions work, dilutions work w 329, dilution name chem work 15 5, dilutions work, dilution work answers, chemistry dilution practice, dilutions work name key, solutions work 2 molarity and dilution problems answers. It is a common practice to determine microbial counts for both liquid and solid specimenssuspensions of e. Test your knowledge of the properties of serial dilution by using this interactive quiz. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then being a 110 dilution of the previous one in the series. Once you find your worksheet, click on popout icon or print icon to worksheet to print or. Serial dilutions a serial dilution is any dilution where the concentration decreases by the same quantity in each successive step.
Some of the worksheets for this concept are dilutions work, dilutions work, dilutions work w 329, dilutions work name key, making dilutions work, solutions work 2 molarity and dilution problems answers, dilution name chem work 15 5, dilution work answers. Spectrophotometry page 7 of 10 single dilutions background stock solutions are constantly diluted in biological lab settings. Easy speed math they dont teach you in school part 1 addition duration. Antibiotic concentration from stock solution calculation. The power point can be used to reveal instructions to the students during the lab. Click on popout icon or print icon to worksheet to print or. Dilutions worksheet i revised sunday, march 24, 20 2 revision 1. A set of serial dilutions is made, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. Bacterial dilutions and a foolproof way to figure them out look at the. All materials were written for and are currently used. When a solution of glucose, c 6h 12o 6, is diluted, the number of moles of the solute in the original solution is greater than, less than, the same as the number of moles of solute in the resulting less concentrated solution. To determine the concentration you multiply the number of colonies by the inverse of the dilution factor in flask b it was 104. What molarity would the potassium nitrate solution need to be if you were only to need 2. Learn serial dilutions with free interactive flashcards.
Dilutions worksheet w 329 everett community college. Sep 21, 2011 a serial dilution is the stepwise dilution of a substance in solution. Commonly the dilution aspect at every step is regular, leading to a geometric progression of the awareness in a logarithmic fashion. Good practice for those who have never used a syringe too. You multiply the original concentration by the dilution factors for each dilution. In the lab, correct dilutions of solutions are critical to performing accurate science. Worksheets are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. Students complete prelab and postlab questions and data table. Note that serial dilution is a special case of series of dilutions. Dilutions worksheet i revised sunday, march 24, 20 1 revision 1.
Jan 10, 2011 a serial dilution is the stepwise dilution of a substance in solution. Concentrations and dilutions answer key kiddy math. Dilutions part 3 of 4 calculating colony forming unitsml duration. If one ml was spread on plate a it would be x 10 4 or 000 or 1. Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. Dilutions worksheet 1 if i add 25 ml of water to 125 ml of a 0. A serial dilution is any dilution where the concentration decreases by the same quantity in each successive step. Dilutions worksheet solutions 1 if i add 25 ml of water to 125 ml of a 0. Thus, a series of 5, one to ten dilutions equals a one to one hundred thousand dilution. Choose from 8 different sets of serial dilution flashcards on quizlet. Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively. Answers serial dilutions practice worksheet biol 307 studocu.
The following problem sets test your ability to calculate dilution factors and concentration s. Mi x v, mol useful kquations mi x v, m2 x v2 molarity molsolutc l. This quiz and attached worksheet will help gauge your understanding of the properties of serial dilution. Pltw, mi unit 1 serial dilution practice quiz quizizz. Serial dilution displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. When youre thinking about dilution, it helps to simplify your actions into dilution factors. What does this mean if a solution has a 110 dilution the number represents 1 part of the patient sample added to 9 parts of diluent. Utilize the worksheet to identify the more important study.
When we said the diluted coffee was 110th as strong as the original that was a dilution factor. Concentrations and dilutions answer key displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work, dilutions work name key, dilutions work w 329, concentrations and dilutions, molarity and serial dilutions teacher handout, laboratory math ii solutions and dilutions. Serial dilution worksheet bio152 paramedical micro. Displaying all worksheets related to serial dilution.
From the original culture 1 ml of broth was transferred to flask a which contains 99 ml of sterile water. The second type gives you the number of bacteria per ml in the original. Showing top 8 worksheets in the category serial dilution. Students perform serial dilution of food coloring experiment with partner, examine concentration levels, and differentiate parts per million ppm and parts per billion ppb. Serial dilution worksheet bio152 paramedical microbiology 1. I made this for my as biology students who dont take chemistry and needed some practice with serial dilutions before jumping straight into a full practical. How to work microbiology dilution problems sciencing. A solution can be made less concentrated in a process called dilution. The first type gives you the number of bacterial colonies found on a plate and asks for the number of bacteria per ml in the original culture. Serial dilutions austin community college district. Some of the worksheets displayed are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. Topics you will need to know in order to pass the quiz.
In a serial dilution, the final total dilution is a product of each individual dilution in the series. Use the following example of a series of dilutions and plating to answer the problems below. Once you find your worksheet, click on popout icon or print icon to worksheet to print or download. Dilution worksheet and problems biology libretexts. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step in serial dilutions, you multiply the dilution factors for each step. In medicine, these skills are necessary to ensuring proper medicine dosage and treatment. Determine the concentration or dilution factor for the flasks and plates. Discovering ecosystems cary institute of ecosystem studies. The answers i sure hope you tried to answer the problems before you looked here. Some of the worksheets displayed are dilutions work, dilutions work, dilutions work w 329, dilutions work name key, making dilutions work, solutions work 2 molarity and dilution problems answers, dilution name chem work 15 5, dilution work answers. We worked through the first example together on the board and the students did the dilution using coloured water. Most questions you will be asked to answer about serial dilutions are of two types. Serial dilutions reduce the concentration of a sample in small steps or fractions.
Concentrations and dilutions answer key worksheets kiddy. Upon completion of this lab, the student should be able to proficiently calculate molarities for solutions. Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. This is called a dilution series or a serial dilution. It is critical that you master both serial and simple single dilutions. We could also have said the dilution factor was 110, or the dilution factor was 0. Serial dilutions practice problems san diego miramar college. Dilutions worksheet w 329 everett community college student support services program 1 if 45 ml of water are added to 250 ml of a 0. This general microbiology practical lecture explains the serial dilution techniques in pour plate method to isolate bacteria. Serial dilution practice problem set science primer. Molarity and serial dilutions teacher handout module overview one of the most important skills in science is to calculate dilutions. A serial dilution is a stepwise dilution of a substance in solution. Dilutions worksheet socorro independent school district.
287 78 697 995 9 358 1512 477 14 534 116 108 7 1294 1310 1089 1138 1556 1422 740 1056 620 1352 9 1178 858 1387 203 1304 1476 433 420 1275 1129 284 1399 887